Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Impaired wound healing with defective expression of chemokines and recruitment of myeloid cells in TLR3-deficient mice.
doi: 10.4049/jimmunol.1003007
Figure Lengend Snippet: FIGURE 5. Chemokine expression in skin wounds. A, Real-time PCR analysis of chemokine mRNA expres- sion in wounds on days 1, 3, and 5 postinjury in WT and TLR32/2 mice. The relative changes were normalized against the mRNA of GAPDH and calculated using the 22DDCT method. Results were from three separate ex- periments performed in duplicate and expressed as mean 6 SEM of fold increase over control. *p , 0.05 in- dicates significantly greater values in WT mice in comparison with the baseline in TLR32/2 mice. B–D, The protein levels of MIP-2/CXCL2 (B), MIP-1a/CCL3 (C), and MCP-1/ CCL2 (D) in the wounds of WT and TLR32/2 mice measured by ELISA. Values represent mean 6 SEM of du- plicate determinations. *p , 0.05 in- dicates significantly greater values in WT mice. Representative results from three independent experiments with four animals in each group are shown.
Article Snippet: Quick start Bradford protein assay ELISA kit was from Bio-Rad Laboratories (Hercules, CA).
Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Comparison, Enzyme-linked Immunosorbent Assay